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isotype control  (R&D Systems)


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    R&D Systems isotype control
    Isotype Control, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+igg1+isotype+control/Rat+IgG1+Isotype+Control/pm41922900-292-13-20
    Average 94 stars, based on 316 article reviews
    isotype control - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Control:

    Article Title: G-CSF Mediates Increased Renal Neutrophils and Kidney Damage in a Psoriasis Mouse Model
    Article Snippet: Complete blood counts were performed on whole blood using a Forcyte Analyzer (Oxford Science, Inc) in the Vanderbilt University Translational Pathology Shared Resource. .. KC-Tie2 and littermate control mice starting at 9 weeks of age were injected intraperitoneally with anti-G-CSF neutralizing antibodies (25 μg per injection; R&D Systems MAB414) or rat IgG1 isotype control (25 μg per injection; R&D Systems MAB005) every two to three days for up to three times per week. ..

    Article Title: Mycobacterium tuberculosis-specific T cells restrain anti-cancer drug-induced neutrophilic lung inflammation in tuberculosis.
    Article Snippet: Neutrophil depletion: 250μg of anti-Ly6G (1A8; Bio X cell, Lebanon, NH, USA) or rat IgG2a isotype control (2A3; Bio X cell) were administered to Mtb-infected mice at 14, 16, 18 days post Mtb infection via intraperitoneal route. .. G-CSF neutralization: 50μg of anti-G-CSF antibody (67604; R&D systems) or rat IgG1 isotype control (43414; R&D systems) were administered to mice starting at 13 days post infection, every alternate day till day 27 via intraperitoneal route. .. IFN-γ neutralization: 250μg of anti-IFN-γ (XMG1.2; Bio X cell) or rat IgG1 isotype control (HRPN; Bio X cell) were administered to Mtb-infected mice starting at 6 days post infection, every alternate day till day 16. via intraperitoneal route.

    Article Title: Type I IFNs contribute to upregulation of PD-L1 during Chlamydia trachomatis infection.
    Article Snippet: Recombinant IFN-α and IFN-β Naïve MEFs were incubated with active recombinant mouse IFN-α (Abcam) or IFN-β (R&D Systems) where indicated for 6 h prior to RT-qPCR or flow cytometry analysis. .. Thawed filter-sterilized supernatants from infected MEFs were incubated with rat IgG1 Isotype control (R&D Systems), rabbit IgG control (R&D Systems), anti-IFN-α (PBL Assay Science), or anti-IFN-β (R&D Systems) for 1 h prior to transfer onto naïve MEFs. ..

    Article Title: Enhanced Apc Min/+ adenoma formation after epithelial CUL4B deletion by recruitment of myeloid-derived suppressor cells.
    Article Snippet: .. Recombinant murine G-CSF (mG-CSF, PeproTech, 250-05, 100 ng/mL), neutralizing antibody of G-CSF (anti-G-CSF, R&D systems, MAB414, 0.5 μg/mL) and Rat IgG1 isotype control (IgG, R&D systems, MAB005, 0.5 μg/mL) were added. .. After incubating for 48 h, the slides were washed with PBS and fixed in ice-cold Immunol Staining Fix Solution (Beyotime, P0098) for 30 min.

    Article Title: Mycobacterium tuberculosis -specific T cells restrain anti-cancer drug-induced neutrophilic lung inflammation in tuberculosis
    Article Snippet: Neutrophil depletion: 250 μg of anti-Ly6G (1A8; Bio X cell, Lebanon, NH, USA) or rat IgG2a isotype control (2A3; Bio X cell) were administered to Mtb-infected mice at 14, 16, 18 days post Mtb infection via intraperitoneal route. .. G-CSF neutralization: 50 μg of anti-G-CSF antibody (67604; R&D systems) or rat IgG1 isotype control (43414; R&D systems) were administered to mice starting at 13 days post infection, every alternate day till day 27 via intraperitoneal route. .. IFN-γ neutralization: 250 μg of anti-IFN-γ (XMG1.2; Bio X cell) or rat IgG1 isotype control (HRPN; Bio X cell) were administered to Mtb-infected mice starting at 6 days post infection, every alternate day till day 16. via intraperitoneal route.

    Article Title: Targeting tumor-intrinsic BCL9 reverses immunotherapy resistance by eliciting macrophage-mediated phagocytosis and antigen presentation.
    Article Snippet: In some experiments, the soluble BMP4 present in the coculture system was neutralized by a rat anti-mouse BMP-4 monoclonal ab (R&D Systems, MAB50201). .. A rat IgG1 isotype control (MAB005, R&D Systems) was used as a negative control. ..

    Article Title: Type I IFNs contribute to upregulation of PD-L1 during Chlamydia trachomatis infection
    Article Snippet: Naïve MEFs were incubated with active recombinant mouse IFN-α (Abcam) or IFN-β (R&D Systems) where indicated for 6 h prior to RT-qPCR or flow cytometry analysis. .. Thawed filter-sterilized supernatants from infected MEFs were incubated with rat IgG1 Isotype control (R&D Systems), rabbit IgG control (R&D Systems), anti-IFN-α (PBL Assay Science), or anti-IFN-β (R&D Systems) for 1 h prior to transfer onto naïve MEFs. ..

    Article Title: Targeting tumor-intrinsic BCL9 reverses immunotherapy resistance by eliciting macrophage-mediated phagocytosis and antigen presentation
    Article Snippet: In some experiments, the soluble BMP4 present in the coculture system was neutralized by a rat anti-mouse BMP-4 monoclonal ab (R&D Systems, MAB50201). .. A rat IgG1 isotype control (MAB005, R&D Systems) was used as a negative control. ..

    Injection:

    Article Title: G-CSF Mediates Increased Renal Neutrophils and Kidney Damage in a Psoriasis Mouse Model
    Article Snippet: Complete blood counts were performed on whole blood using a Forcyte Analyzer (Oxford Science, Inc) in the Vanderbilt University Translational Pathology Shared Resource. .. KC-Tie2 and littermate control mice starting at 9 weeks of age were injected intraperitoneally with anti-G-CSF neutralizing antibodies (25 μg per injection; R&D Systems MAB414) or rat IgG1 isotype control (25 μg per injection; R&D Systems MAB005) every two to three days for up to three times per week. ..

    Neutralization:

    Article Title: Mycobacterium tuberculosis-specific T cells restrain anti-cancer drug-induced neutrophilic lung inflammation in tuberculosis.
    Article Snippet: Neutrophil depletion: 250μg of anti-Ly6G (1A8; Bio X cell, Lebanon, NH, USA) or rat IgG2a isotype control (2A3; Bio X cell) were administered to Mtb-infected mice at 14, 16, 18 days post Mtb infection via intraperitoneal route. .. G-CSF neutralization: 50μg of anti-G-CSF antibody (67604; R&D systems) or rat IgG1 isotype control (43414; R&D systems) were administered to mice starting at 13 days post infection, every alternate day till day 27 via intraperitoneal route. .. IFN-γ neutralization: 250μg of anti-IFN-γ (XMG1.2; Bio X cell) or rat IgG1 isotype control (HRPN; Bio X cell) were administered to Mtb-infected mice starting at 6 days post infection, every alternate day till day 16. via intraperitoneal route.

    Article Title: Mycobacterium tuberculosis -specific T cells restrain anti-cancer drug-induced neutrophilic lung inflammation in tuberculosis
    Article Snippet: Neutrophil depletion: 250 μg of anti-Ly6G (1A8; Bio X cell, Lebanon, NH, USA) or rat IgG2a isotype control (2A3; Bio X cell) were administered to Mtb-infected mice at 14, 16, 18 days post Mtb infection via intraperitoneal route. .. G-CSF neutralization: 50 μg of anti-G-CSF antibody (67604; R&D systems) or rat IgG1 isotype control (43414; R&D systems) were administered to mice starting at 13 days post infection, every alternate day till day 27 via intraperitoneal route. .. IFN-γ neutralization: 250 μg of anti-IFN-γ (XMG1.2; Bio X cell) or rat IgG1 isotype control (HRPN; Bio X cell) were administered to Mtb-infected mice starting at 6 days post infection, every alternate day till day 16. via intraperitoneal route.

    Infection:

    Article Title: Mycobacterium tuberculosis-specific T cells restrain anti-cancer drug-induced neutrophilic lung inflammation in tuberculosis.
    Article Snippet: Neutrophil depletion: 250μg of anti-Ly6G (1A8; Bio X cell, Lebanon, NH, USA) or rat IgG2a isotype control (2A3; Bio X cell) were administered to Mtb-infected mice at 14, 16, 18 days post Mtb infection via intraperitoneal route. .. G-CSF neutralization: 50μg of anti-G-CSF antibody (67604; R&D systems) or rat IgG1 isotype control (43414; R&D systems) were administered to mice starting at 13 days post infection, every alternate day till day 27 via intraperitoneal route. .. IFN-γ neutralization: 250μg of anti-IFN-γ (XMG1.2; Bio X cell) or rat IgG1 isotype control (HRPN; Bio X cell) were administered to Mtb-infected mice starting at 6 days post infection, every alternate day till day 16. via intraperitoneal route.

    Article Title: Type I IFNs contribute to upregulation of PD-L1 during Chlamydia trachomatis infection.
    Article Snippet: Recombinant IFN-α and IFN-β Naïve MEFs were incubated with active recombinant mouse IFN-α (Abcam) or IFN-β (R&D Systems) where indicated for 6 h prior to RT-qPCR or flow cytometry analysis. .. Thawed filter-sterilized supernatants from infected MEFs were incubated with rat IgG1 Isotype control (R&D Systems), rabbit IgG control (R&D Systems), anti-IFN-α (PBL Assay Science), or anti-IFN-β (R&D Systems) for 1 h prior to transfer onto naïve MEFs. ..

    Article Title: Mycobacterium tuberculosis -specific T cells restrain anti-cancer drug-induced neutrophilic lung inflammation in tuberculosis
    Article Snippet: Neutrophil depletion: 250 μg of anti-Ly6G (1A8; Bio X cell, Lebanon, NH, USA) or rat IgG2a isotype control (2A3; Bio X cell) were administered to Mtb-infected mice at 14, 16, 18 days post Mtb infection via intraperitoneal route. .. G-CSF neutralization: 50 μg of anti-G-CSF antibody (67604; R&D systems) or rat IgG1 isotype control (43414; R&D systems) were administered to mice starting at 13 days post infection, every alternate day till day 27 via intraperitoneal route. .. IFN-γ neutralization: 250 μg of anti-IFN-γ (XMG1.2; Bio X cell) or rat IgG1 isotype control (HRPN; Bio X cell) were administered to Mtb-infected mice starting at 6 days post infection, every alternate day till day 16. via intraperitoneal route.

    Article Title: Type I IFNs contribute to upregulation of PD-L1 during Chlamydia trachomatis infection
    Article Snippet: Naïve MEFs were incubated with active recombinant mouse IFN-α (Abcam) or IFN-β (R&D Systems) where indicated for 6 h prior to RT-qPCR or flow cytometry analysis. .. Thawed filter-sterilized supernatants from infected MEFs were incubated with rat IgG1 Isotype control (R&D Systems), rabbit IgG control (R&D Systems), anti-IFN-α (PBL Assay Science), or anti-IFN-β (R&D Systems) for 1 h prior to transfer onto naïve MEFs. ..

    Incubation:

    Article Title: Type I IFNs contribute to upregulation of PD-L1 during Chlamydia trachomatis infection.
    Article Snippet: Recombinant IFN-α and IFN-β Naïve MEFs were incubated with active recombinant mouse IFN-α (Abcam) or IFN-β (R&D Systems) where indicated for 6 h prior to RT-qPCR or flow cytometry analysis. .. Thawed filter-sterilized supernatants from infected MEFs were incubated with rat IgG1 Isotype control (R&D Systems), rabbit IgG control (R&D Systems), anti-IFN-α (PBL Assay Science), or anti-IFN-β (R&D Systems) for 1 h prior to transfer onto naïve MEFs. ..

    Article Title: Type I IFNs contribute to upregulation of PD-L1 during Chlamydia trachomatis infection
    Article Snippet: Naïve MEFs were incubated with active recombinant mouse IFN-α (Abcam) or IFN-β (R&D Systems) where indicated for 6 h prior to RT-qPCR or flow cytometry analysis. .. Thawed filter-sterilized supernatants from infected MEFs were incubated with rat IgG1 Isotype control (R&D Systems), rabbit IgG control (R&D Systems), anti-IFN-α (PBL Assay Science), or anti-IFN-β (R&D Systems) for 1 h prior to transfer onto naïve MEFs. ..

    Recombinant:

    Article Title: Enhanced Apc Min/+ adenoma formation after epithelial CUL4B deletion by recruitment of myeloid-derived suppressor cells.
    Article Snippet: .. Recombinant murine G-CSF (mG-CSF, PeproTech, 250-05, 100 ng/mL), neutralizing antibody of G-CSF (anti-G-CSF, R&D systems, MAB414, 0.5 μg/mL) and Rat IgG1 isotype control (IgG, R&D systems, MAB005, 0.5 μg/mL) were added. .. After incubating for 48 h, the slides were washed with PBS and fixed in ice-cold Immunol Staining Fix Solution (Beyotime, P0098) for 30 min.

    Negative Control:

    Article Title: Targeting tumor-intrinsic BCL9 reverses immunotherapy resistance by eliciting macrophage-mediated phagocytosis and antigen presentation.
    Article Snippet: In some experiments, the soluble BMP4 present in the coculture system was neutralized by a rat anti-mouse BMP-4 monoclonal ab (R&D Systems, MAB50201). .. A rat IgG1 isotype control (MAB005, R&D Systems) was used as a negative control. ..

    Article Title: Targeting tumor-intrinsic BCL9 reverses immunotherapy resistance by eliciting macrophage-mediated phagocytosis and antigen presentation
    Article Snippet: In some experiments, the soluble BMP4 present in the coculture system was neutralized by a rat anti-mouse BMP-4 monoclonal ab (R&D Systems, MAB50201). .. A rat IgG1 isotype control (MAB005, R&D Systems) was used as a negative control. ..



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